Turun Yliopisto 0245896-3 Yliopistonmäki Turku 20014 Jaakko Uotila +358 503839896 procurement@utu.fi http://www.utu.fi SIM/TIRF imaging system. 40/02.05.03/2016 The Cell Imaging Core (CIC) is a major service provider of microscopy platforms in Turku. CIC has taken on the responsibility to provide super-resolution technologies, as part of Euro-BioImaging (EuBI). As a managing and imaging site of the Finnish Advanced Light Microscopy Node of EuBI, Turku provides services at international and national level, and it has committed to provide multiple imaging services with a specialty in super-resolution light microscopy. Turku provides STED microscopy services and develops imaging and analysis methods for STED and other super-resolution methods. CIC intends to purchase an imaging system with Structured Illumination Microscopy (SIM) and Total Internal Reflection Microscopy (TIRF), which is optimized for high and super-resolution live cell imaging. The combination of STED and SIM offers a full platform to investigate biomedical samples with super-resolution. SIM will also work as a reference method to STED in research and application development. 308000.00 The microscope has to be optimized for fast and quantitative imaging, and has to have modules to do photomanipulation, simultaneous FRET, and simultaneous TIRF imaging and single molecule tracking of 3 fluorescence channels (3 cameras). The system must have 6 laser lines (nm): 405, 445, 488, 514, ~568, ~642, all the lines minimum 100 mW allowing single molecule detection. TIRF must be based on ring-TIRF illumination to do quantitative imaging over a full field of view. The microscope has to improve resolution in 3D (XYZ) by using common fluorescent labels and 1-3 super-resolution channels at the same time. 3D SIM has to achieve resolution of (XYZ): 110/310 nm at 405 nm, 115/310 nm at 488 nm, 130/330 nm at 568 nm, 160/370 nm at 641 nm. SIM imaging speed must be 15 fps in 2D and 1 fps in 3D. Common requirements: — Microscope stage with a 100 nm step accuracy (XY), 0.010 mm repeatability; — Piezo Z-focus system with a 5 nm resolution; — Laser based autofocus device; — Three CMOS cameras, FOV: 512x512 SIM and 1024x1024 TIRF; — Incubation system for live cells, and sample holders; — Air damped optical table; — Isolation cabin over the whole microscope; — Full warranty for minimum 1 year. CIC has decided to buy a second hand GE DeltaVision OMX imaging system due to its unique hardware design, which is needed especially in the cell biological research in Turku. Only the DeltaVision OMX can fulfil the unique feature requirements that we have for the system (see below). 1) SIM features: Imaging speed must be: — 1 fps or faster in 3D SIM mode (super-resolution in XY and Z in live cell experiments); — 15 fps or faster in 2D SIM mode. This imaging speed can be achieved in SIM only with a blaze-based structured illumination. DeltaVision OMX is the only system that can image faster than 10 frames per second in 2D. Laboratory of Prof. Ivaska, University of Turku, has performed experiments using a blaze-based SIM at the Janelia Research Campus, US in 2016, which revealed advantages of a high speed hardware in live cell imaging. The laboratories of Prof. Ivaska and Dr. Mattila, University of Turku, both investigate highly dynamic cellular processes, including vesicle transport and actin cytoskeletal dynamics. For example, in Mattila group's ongoing experiments, reliable tracking of small antigen containing endocytic vesicles in B lymphocytes requires acquisition at the minimum frame rate of 10 fps. Also, a big part of early endosomes remain undetected at conventional resolution (revealed by STED). To reliably follow these very small and dynamic intracellular vesicles requires fast super-resolution imaging in living cells. The laboratory of Dr. Coffey, Åbo Akademi University, requires high speed super-resolution (x,y,z) SIM blaze for imaging of the very fast motility of actin in dendritic spines (i.e. neuronal synapses <0.15 µm). 2) TIRF features: — Ring TIRF illumination: to create an uniform TIRF illumination over the whole field of view (1024x1024), which is needed for quantitative analysis. Ring TIRF also significantly enhances the amount of high resolution data obtained per field of view. — 3-camera system aligned and calibrated on pixel level for simultaneous TIRF imaging of 3 colours at ensemble or at single molecule level in live cells. 3) The system must include an exact calibration protocol to control channel alignment at pixel level. 4) Linux OS system (transparent, open source code for method development). This avoids premature redundancy of instrument as occurs with Windows-based systems where software become quickly obsolete and backwards compatibility is not guaranteed by vendors beyond a 5-year period. SIM/TIRF imaging system 2016-12-14 GE Healthcare Europe GmbH Freiburg 308000.00 Markkinaoikeus Radanrakentajantie 5 Helsinki 00520 +358 295643300 markkinaoikeus@oikeus.fi +358 295643314 http://www.oikeus.fi/markkinaoikeus 2016-12-19
See tender at TED: http://ted.europa.eu/udl?uri=TED:NOTICE:451423-2016:TEXT:EN:HTML