Assignment text
The instrument will be in the department of Chemistry in the group of Nikos Hatzakis and will be involved in the center for 4D cellular dynamics
and the challenge center for optimized oligo escape. The main scope of this work is to observe in real time and in 4D (3D and time) live cells and how ligands or nanocarriers, or Lipid nanoparticles with oligonucleotides or other nanomaterials are entering and being delivered in cells. A central part of the work is parallel recording of multiple cells, biocompatibility with imaging slides and using as little volume of material as possible.
The intended procurement is Zeiss lattice light-sheet microscopy. It is an automated, and easy-to-use system, allowing live cell imaging at subcellular resolution with best protection from photo damage.
The adaptability of the instrument allows using multicell well plates (up to 96 well plates) offering parallelized imaging at diverse conditions. We will use this microscopy to study cell dynamics in 4D. Meanwhile, it will be open to other research group, allowing researchers to volumetric imaging of subcellular structures and dynamics.